Part:BBa_J64100:Design

Medium copy ColE1 BioBrick vector

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found at 124
  Illegal suffix found at 821
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 124
  Illegal SpeI site found at 822
  Illegal PstI site found at 836
  Illegal NotI site found at 130
  Illegal NotI site found at 829
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 124
  Illegal BamHI site found at 809
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 124
  Illegal suffix found at 822
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 124
  Plasmid lacks a suffix.
  Illegal XbaI site found at 139
  Illegal SpeI site found at 822
  Illegal PstI site found at 836
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 259
  

Design Notes

None

Source

pProTetE333 (clontech) and biobricks parts

References

<biblio>

1. Bazaral-J-Mol-Biol-1968 pmid=4939624
2. Hershfield-Proc-Natl-Acad-Sci-USA-1974 pmid=4610576

</biblio>